Aberrant c-myc RNAs of Burkitt's lymphoma cells have longer half-lives

摘要: BL67 and BL18 are Burkitt's lymphoma cell lines with t(8;14) translocations (the breakpoint is in the first exon intron, respectively) which mu-heavy chain switch region fused to c-myc gene head orientation. In both only aberrant RNAs found. cells contain two RNA species of 2.4 3.5 kb. The 2.4-kb initiated at several cryptic promoters intron. 3.5-kb transcribed from immunoglobulin heavy anti-sense strand across translocation into then normally spliced using physiological splice donor acceptor sites gene. contains kb intron additional 0.90 0.74 dual on reciprocal fragment translocation. cytoplasmic turnover these differs significantly that normal message. 0.90-kb 0.74-kb cells, hybrid molecules consisting sequences, have a half-life hours contrast message 15 min. also more stable than disappear 50 min.(ABSTRACT TRUNCATED AT 250 WORDS)