The matrix effect in particle beam liquid chromatography/mass spectrometry and reliable quantification by isotope dilution.

摘要: The transport efficiency of the particle beam liquid chromatography/mass spectrometer interface is influenced by analyte concentration contributing to a widely reported non-linearity. In this work, coeluting, isotope-labeled internal standards were investigated as 'carriers' improve and linearity. Three styrene metabolites--mandelic, phenylglyoxylic hippuric acids--and their pentadeutero analogs separated reversed-phase chromatography (LC) with an ammonium acetate-acetonitrile mobile phase. Selected positive ions produced electron ionization monitored generate LC/MS calibration curves. present study demonstrates that not only non-linear, but also subject matrix effect presumably same mechanism responsible for Coeluting, ineffective at linearizing chromatograph/mass detector response. Isotope dilution quantification, however, compensates variable efficiencies, linearizes effect, affording reliable quantification metabolites.