Mechanism of action and fate of the fungicide chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) in biological systems: I. Reactions with cells and subcellular components of Saccharomyces pastorianus

摘要: Abstract The mechanism and sequence of reaction chlorothalonil in cells Saccharomyces pastorianus was investigated by the use either 14C-labeled fungicide or Na235SO4-labeled cells. initial uptake resulted rapid formation substituted chlorothalonil-reduced glutathione (GSH) derivatives. Chlorothalonil reacted with proteins during derivative but decreased cell viability did not occur until all GSH inhibition specific NAD thiol-dependent glycolytic respiratory enzymes occurred. It is postulated that enzyme activity are controlled directly indirectly concentration GSH. loss through lack regeneration reduced thiol could be an important feature toxicity fungicide. general action resembles trichloromethyl sulfenyl fungicides treated accumulate large concentrations fungicide; reactions involve both low high molecular-weight thiols, glutathione-fungicide derivatives; resides ultimately enzymes.