New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions
作者： Anke Becker , Michael Schmidt , Wolfgang Jäger , Alfred Pühler
DOI: 10.1016/0378-1119(95)00313-U
关键词:
摘要: A set of antibiotic-resistance and promoter-probe cassettes suitable for insertion mutagenesis generation transcriptional fusions was constructed. The contain the aacC1 gene transposon Tn1696 conferring resistance to gentamicin in a large variety Gram- Gram+ bacteria. In addition gene, promoterless Escherichia coli lacZ included cassettes, allowing determination activity at site. can be excised from plasmid mediating ampicillin by many commonly used restriction enzymes. new constructs have been successfully studies transcription Rhizobium meliloti.
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