High-Level Expression of Angiogenic Factors Is Associated with Advanced Tumor Stage in Human Neuroblastomas

摘要: Angiogenesis is essential for tumor growth and metastasis depends on the production of angiogenic factors by cells. Neuroblastoma (NB) a common pediatric neural crest origin, which biologically clinically heterogeneous. Increased vascular index correlates with poor outcome NB. To determine contribute to NB angiogenesis thereby support progression, we examined expression eight [vascular endothelial factor (VEGF), VEGF-B, VEGF-C, basic fibroblast factor, angiopoietin (Ang)-1, Ang-2, transforming alpha, platelet-derived (PDGF)] semiquantitative RT-PCR in 37 primary tumors 22 cell lines. We also analyzed relationship between clinicopathological as well patient survival. All were expressed at various levels lines tumors, suggesting their involvement angiogenesis. The most correlated each other, synergy regulating process. Significantly higher VEGF, PDGF-A (P < 0.0001-0.026) found advanced-stage (stages 3 4) compared low-stage 1, 2, 4S). Expression was significantly associated survival = 0.04). redundancy suggests that inhibition VEGF bioactivity alone might not be sufficient approach antiangiogenic therapy human