Expression of Vascular Permeability Factor (Vascular Endothelial Growth Factor) and Its Receptors in Adenocarcinomas of the Gastrointestinal Tract

摘要: Vascular permeability factor (VPF) is one of the most potent known inducers microvascular hyperpermeability; in addition, it a selective endothelial cell growth factor, hence its alternate name, vascular factor. VPF exerts actions on microvasculature by interacting with specific receptors. expressed many transplantable animal tumors, tumor lines culture, and certain normal cells situ. The purpose present investigation was to determine whether what consistency receptors are primary autochthonous human gastrointestinal tract origin, as determined situ hybridization immunohistochemistry. Twenty-one adenocarcinomas (17 colon, 2 stomach, 1 small bowel, pancreas) were studied. malignant epithelial mRNA strongly, contrast epithelium, hyperplastic polyps, adenomas, which little or no mRNA. expression further increased immediately adjacent zones necrosis; such areas, occasional stromal also In ten colon carcinomas studied, stained for protein nearby blood vessels immunohistochemistry addition mRNAs encoding flt-1 kdr hybridization. Endothelial away from did not stain definite detected ganglion myenteric plexus bowel protein. These data indicate that tumors origin regularly express both likely contribute promoting angiogenesis stroma formation, directly, through action an indirectly, increasing permeability, thereby leading plasma extravasation, fibrin deposition, eventual replacement resulting matrix vascularized stroma.