作者: Gernot Schabbauer , Michael Tencati , Brian Pedersen , Rafal Pawlinski , Nigel Mackman
DOI: 10.1161/01.ATV.0000143096.15099.CE
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摘要: … For macrophage and E-selectin staining, antigen retrieval was performed using trypsin digestion in phosphate-buffered saline with 10 mmol/L CaCl 2 at 37C for 5 minutes. Tissue …