Verapamil reversal of vincristine resistance and cross-resistance patterns of vincristine-resistant Chinese hamster ovary cells.

摘要: The phenotypic properties of a large number low-level vincristine (VCR)-resistant Chinese hamster ovary cell lines have been studied. In particular, we examined the correlation between reversal VCR resistance by verapamil (VRP) and cross-resistance patterns to other drugs. Cross vinca alkaloids developed in parallel with resistance. Doxorubicin was observed many lines, occurring even at lowest levels Increased sensitivity taxol (TAX) feature half tested there no increased 5-fluorouracil or chlorambucil. Two main groups mutants identified: (a) those that are thought be membrane which cross-resistant TAX whose is reversible VRP; (b) appear tubulin mutants, presence VRP. It not possible distinguish different mechanisms drug accumulation studies unsupplemented medium. However, VRP did correlate results.