17Beta-estradiol antagonizes effects of 1alpha,25-dihydroxyvitamin D3 on interleukin-6 production and osteoclast-like cell formation in mouse bone marrow primary cultures.

摘要: In mouse bone marrow primary cultures, the formation of osteoclast-like, i.e. tartrate-resistant acid phosphatase (TRAP)- and calcitonin receptor-positive multinucleated cells (MNC), when induced by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3), can be suppressed 17β-estradiol (17β-E2), whereas 17α-E2 is without any effect. 17β-E2, above 10−11 m, significantly reduced 1α,25(OH)2D3-mediated TRAP+ MNC in cultured from both female male mice. The estrogen at 10−8 m peak response to vitamin D sterol 50%. 17β-E2 basal 1α,25(OH)2D3-stimulated cellular production interleukin (IL)-6. IL-6 alone, although hormone-free culture produced appreciable amounts cytokine, did not induce MNC. Therefore, changes hormones could sole determinant for extent formation. However, stimulatory effect 1α,25(OH)2D3 on osteoclastogenesis nevert...