Bio-Control of Alternaria alternata during Banana Storage by Purified AFP Using Isoelectric Focusing Technique

摘要: Interestingly, antifungal protein AFP was purified from Aspergillus giganteus supernatants with modified isoelectric focusing procedure after adaptation of the secretion conditions. Subsequently, activity as well mode action against Alternaria alternata was tested in vitro. Moreover, different concentrations were applied to banana fruits for 15 days at 20°C vivo. Obtained results illustrated that A. able secrete about 39.78 ± 2.39 mg AFP·l-1 Olson medium. The employed ammonium sulfate (AS 75%) precipitation followed by dialysis steps yielded 16 - 22 culture supernatant with general mean 18.67 1.98 mg·l-1. lost amount during purification using AS and 3KDa cut-off membrane is 50% thus, purification procedure must be further improved. Indeed, concluded results MIC hyphal extension inhibition test noticed was efficiently affected either growth or hyphae form against 2μg·ml-1. However,short, thick highly septated with damaged constricted apical regions extruding condensed mycelium aggregates treated compared to untreated remarkably shown. action vitro experiment manifested AFP was effective act fungal cell permeabilize alternata. Furthermore, vivo showed could reduce post-harvest decay on caused A. alternata. concentration 25 μg·ml-1 exhibit decayed reduction 45.45% 77.27%, respectively. While no area observed when 50 storage time. Quantification DNA by species-specific PCR exude a positive correlation between DNA amount area. In conclusion, can efficiently used a bio-preservative agent handling fruits, and considered an excellent biological alternative combat secondary of filamentous fungi.