Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein
作者： Xu Hu , A.S.N. Reddy
关键词: Arabidopsis 、 Amino acid 、 Molecular biology 、 Biology 、 Signal peptide 、 Complementary DNA 、 Gene 、 Southern blot 、 Peptide sequence 、 Pathogenesis-related protein 、 Biochemistry
摘要: Pathogenesis-related (PR)-5 proteins are a family of that induced by different phytopathogens in many plants and share significant sequence similarity with thaumatin. We isolated complementary DNA (ATLP-3) encoding PR5-like protein from Arabidopsis which is distinct two other previously reported PR5 cDNAs the same plant species. The predicted ATLP-3 its amino-terminal signal 245 amino acids length acidic pI 4.8. deduced acid shows thaumatin-like contains putative at amino-terminus. expression related gene (ATLP-1) we was pathogen infection salicylic acid, known inducer pathogenesis-related genes. Southern blot analysis indicates ATLP-1 coded single-copy To study effect on fungal growth, cDNA regions corresponding to mature were expressed Escherichia coli encoded purified. cross-reacted anti-osmotin anti-zeamatin antibodies. showed antifungal activity against several pathogens suggesting may be involved defense pathogens.
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