Investigation of volatile organic metabolites in lung cancer pleural effusions by solid-phase microextraction and gas chromatography/mass spectrometry

摘要: Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/mass spectrometry (GC/MS) method was applied for the investigation of low molecular weight volatile organic metabolites (VOMs) in pleural effusion samples. Three important HS-SPME experimental parameters that influence extraction efficiency (fiber coating, time and temperature sampling) were optimized by a univariate optimization design. The highest obtained when sampling performed at 50°C 10min under agitation using carboxen/polydimethylsiloxane (CAR/PDMS) fiber. A total 36 belonging to nine distinct chemical classes identified 40 samples (20 malignant effusions from lung cancer patients 20 benign inflammatory patients). Ketones, alcohols, benzene derivatives main metabolomic profile effusions. average peak areas ketones alcohols much higher group compared group. Together phenols, they exhibit significant differences (P<0.05) between two groups. Particularly, cyclohexanone 2-ethyl-1-hexanol significantly than those ones. Furthermore, metabolites, 5 compounds including found be statistically different (Student's t-test, P<0.05) groups statistical analysis based on all metabolites. Among them, might considered as candidate biomarkers differentiate results show HS-SPME-GC/MS is simple, rapid, sensitive solvent-free determination VOMs Pleural valuable sample source observation changes differentiation individuals.