Gene signature of cancer stem cells in an intrinsic subgroup of breast cancers with mesenchymal properties

摘要: 11009 Background: Breast cancer stem cells characterized by CD44+/CD24−/low may be resistant to therapy and responsible for relapse. Mammospheres (MSs) can propogated as an in vitro surrogate assay increased self-renewal. We set out define a “signature” expression pattern associated with CD44+/CD24−/low, mammosphere-forming cells. Methods: biopsies (n=19) were digested, stained CD24, CD44, lineage antibodies, analyzed flow cytometry. A portion of the unsorted plated under serum-free conditions form MSs (n=16). Gene expression, using Affymetrix U133 GeneChip platform, bearing vs. other sorted cells, between MS primary invasive cancers analyzed. data from two trials (neoadjuvant letrozole N=18, docetaxel, N=12) used validation studies. Results: In flow-sorted 1,424 named genes elevated (p 1.5, FDR=0.20). The ...