Novel effect of the inhibitor of mitochondrial cyclophilin D activation,N-methyl-4-isoleucine cyclosporin, on renal calcium crystallization

摘要: Objectives To experimentally evaluate the clinical application of N-methyl-4-isoleucine cyclosporin, a novel selective inhibitor cyclophilin D activation. Methods In vitro, cultured renal tubular cells were exposed to calcium oxalate monohydrate crystals and treated with cyclosporin. The mitochondrial membrane was stained tetramethylrhodamine ethyl ester perchlorate observed. In vivo, Sprague–Dawley rats divided into four groups: control group, an ethylene glycol group (administration induce crystallization), cyclosporin cyclosporin) glycol + N-methyl-4-isoleucine cyclosporin). Renal crystallization evaluated using Pizzolato staining. Oxidative stress superoxide dismutase 8-hydroxy-deoxyguanosine. Mitochondria within observed by transmission electron microscopy. Cell apoptosis cleaved caspase-3. Results In vitro, induced depolarization potential, which remarkably prevented administration crystallization, oxidative stress, collapse cell in rats, significantly cyclosporin. Conclusions Herein we first report new treatment agent determining through activation.