Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples
作者： Sagarika Deepthy Tallapragada , Keya Layek , Runu Mukherjee , Kalyan Kumar Mistry , Monidipa Ghosh
DOI: 10.1016/J.BIOELECHEM.2017.06.009
关键词:
摘要: In this study, an immunosensor based on screen-printed electrode (SPE) has been developed for the detection of Human Epidermal Growth Factor Receptor-2 (HER2) antigen. The SPEs were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed construction immunosensor. capture antibody (mouse anti-human ErbB2) coated onto surface without any prior modification, by addition recombinant human HER2 Biotinylated goat ErbB2 used as which to streptavidin conjugated horseradish peroxidase (HRP). 3,3',5,5'-tetramethylbenzidine (TMB) substrate. redox reaction measured using cyclic voltammetry at scan rate 50mV/s quantification antigen in solution. biotin-avidin chemistry enabled accurate nanogram levels. amperometric signal obtained increased linearly with increase concentration two-fold linear range between 5ng/ml-20ng/ml 20-200ng/ml respectively. limit (LOD) (LOQ) found be 4ng/ml 5ng/ml serum samples invasive non-invasive breast cancer patients realized.
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