CD27, a member of the nerve growth factor receptor family, is preferentially expressed on CD45RA+ CD4 T cell clones and involved in distinct immunoregulatory functions.

摘要: CD27 is a disulfide-linked 120-kDa homodimer expressed on the majority of peripheral T cells at variable density that belongs to recently defined nerve growth factor receptor family. mAb reactive with can either enhance or inhibit cell activation, suggesting crucial role in process activation. We now show preferentially CD45RA+CD45RO-CD29low subset CD4 cells. expression this maintained for prolonged period culture after PHA In contrast, CD45RA-CD45RO(+)-CD29high express very low level CD27, and its lost within 2 wk To further analyze differential these reciprocal subsets cells, we developed clones by stimulating isolated CD4+CD45RA+ CD4+CD45RO+ populations PHA. derived from originally CD45RA+ retained both CD45RA expression, whereas CD45RO+ were CD45RA- CD27-. functional assays, IL-4 production could only be induced CD45RA-CD27- stimulation PMA ionomycin. Four six had suppressor activity PWM-driven IgG synthesis, five helper activity. Of interest, CD45RA+CD27+ was partially blocked pretreatment anti-CD27 (1A4). Anti-1A4 resulted elevation intracellular cAMP levels. Thus, appears play function may involved least part via effects production.