MicroRNA hsa-miR-29b potentiates etoposide toxicity in HeLa cells via down-regulation of Mcl-1

摘要: Etoposide is commonly used as a monotherapy or in combination with other drugs for cancer treatments. In order to increase the drug efficacy, ceaseless search novel combinations of and supporting molecules under way. MiRNAs are natural candidates facilitating effect various cell types. We several systems evaluate miR-29 family on etoposide toxicity HeLa cells. show that miR-29b significantly increases Because Mcl-1 protein has been recognized target, we evaluated downregulation splicing variant expression induced by precursors confirmed key role enhancing toxicity. Despite all three members, only enhanced hypothesized this difference may be linked change Mcl-1L/Mcl-1S ratio miR-29b. could due nuclear shuttling. Using specifically modified sequences cytosolic localization there difference, albeit statistically non-significant. conclusion, synergistic treatment cells shuttling