Cytokine polymorphic analyses indicate ethnic differences in the allelic distribution of interleukin-2 and interleukin-6.

摘要: BACKGROUND: Polymorphisms in the regulatory regions of cytokine genes affect protein production and are associated with allograft outcome. Ethnic origin has been identified as a significant prognostic factor for several immune-mediated diseases outcome after allotransplantation. A clear relationship between polymorphisms ethnicity not shown. METHODS: One hundred sixty subjects including 102 whites 43 African-Americans were studied. Using polymerase chain reaction-based assays and, some cases, restriction enzyme digestion, we determined genetic cytokines interleukin (IL) -2, IL-6, IL-10, tumor necrosis factor-alpha, transforming growth factor-beta, interferon-gamma (IFN-gamma). Genetic polymorphism frequencies then compared to using chi-square analysis Fisher's exact two-tailed tests. RESULTS: For both IL-2 IL-6 genes, found that differed significantly (P <0.05) their allelic distribution genotype frequency. trend toward ethnic was noted among alleles genotypes IL-10 IFN-gamma genes. We no correlation either or frequency factor-alpha factor-beta When comparisons made patients without history kidney failure, genotypic distributions differ. CONCLUSIONS: Our work demonstrates In addition, requiring renal transplantation differ from general population regard certain gene polymorphisms. These findings may have relevance making determinations tailoring immunomodulatory regimens transplantation.