Exogenous Expression of Interferon-β in Cultured Brain Microvessel Endothelial Cells

摘要: Brain microvessel endothelial cells (BMECs) make up the blood–brain barrier (BBB) and regulate passage of therapeutic proteins as well drugs from cerebrovasucular circulation to brain. In present study, we transferred mouse or human interferon-β (IFN-β) gene via cationic liposomes into primary cultures bovine BMECs developed an in vitro model BBB. The gene-transferred secreted transiently a substantial amount IFN activity more efficiently during growth phase than at confluence. This was suggested be due difference potential for plasmid incorporation between growing confluent series cell association experiments with 32P-labelled DNA. Furthermore, when BMEC monolayers Transwell plates were transfected IFN-β-expression vectors upper side, IFN-β predominantly detected compartments, suggesting polarized secretion transgene products monolayers. These findings provide important basic information about secretory protein delivery BMECs.