Endothelial cell-based cytokine gene delivery inhibits 9L glioma growth in vivo.

摘要: Malignant brain neoplasms present great therapeutic challenges due to their extremely aggressive behavior and relative isolation by the blood-brain blood-tumor barriers. Endothelial cells may be versatile platforms for delivering genes solid tumors virtue of location at blood-tissue interfaces proliferation in response endothelial mitogens produced tumors. Immortalized rat that express E. coli lacZ reporter gene murine interleukin-2 (RBEZ-IL2) were co-inoculated with 9L glioma Fisher rats examine effects cell-based cytokine delivery on growth vivo. was not affected implantation control RBEZ cells. The subcutaneous intracranial gliomas significantly inhibited RBEZ-IL2 (P < 0.005 P 0.001, respectively) when compared transfected Rats receiving showed increased survival 0.001). Histologic immunohistologic analysis enhanced activation microglia/macrophages CD8-positive T lymphocytes and/or natural killer within sites inoculation This report establishes immortalized can used activate anti-tumor host responses experimental central nervous system.