Characterization of 9L glioma model of the Wistar rat.
作者： Maja Stojiljkovic , Vesna Piperski , Mirjana Dacevic , Ljubisav Rakic , Sabera Ruzdijic
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摘要: The aim of our study was to develop and characterize solid brain tumors in Wistar rats, which could be used investigations concerning the molecular mechanisms that lay beneath genesis gliomas as well testing curative potentials various therapeutics. were induced by intracerebral inoculation 9L glioma cells characterized morphometrical, histological immunohistochemical analysis after 7, 14 21 postimplantation days. Immunohistochemical characterization included detection nuclear antigene Ki-67 proliferative cell marker, GFAP a tracer reactive gliosis surrounding tumor mass, CD4/CD8 ED1 antigens, markers immunological response. Our results showed 7 days all experimental animals developed solid, well-circumcised tumors, clearly separated from tissue. Tumors progressive growth 7th 21st day despite observed response starting Histologically hypercellular with neovascularization necrosis. These indicate reproducible morphometric evaluation can performed on growing immunocompetent enabling its use an animal model for therapeutic approaches.
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Sven Gunia, Sami Hussein, Dorel L. Radu, Klaus-Martin Pütz, Roger Breyer, Hartmut Hecker, Madjid Samii, Gerhard F. Walter, Alexandru C. Stan, CD44S-TARGETED TREATMENT WITH MONOCLONAL ANTIBODY BLOCKS INTRACEREBRAL INVASION AND GROWTH OF 9L GLIOSARCOMA Clinical & Experimental Metastasis. ,vol. 17, pp. 221- 230 ,(1999) , 10.1023/A:1006699203287
Marco Saini, Mattia Bellinzona, Frerk Meyer, Gaetano Cali', Madjid Samii, Morphometrical characterization of two glioma models in the brain of immunocompetent and immunodeficient rats. Journal of Neuro-oncology. ,vol. 42, pp. 59- 67 ,(1999) , 10.1023/A:1006128825766
George Morstyn, Andrew H. Kaye, Ian Gardner, Ken Pyke, Development of a xenograft glioma model in mouse brain. Cancer Research. ,vol. 46, pp. 1367- 1373 ,(1986)
D.E. Wilkins, G.P. Raaphorst, J.K. Saunders, G.R. Sutherland, I.C.P. Smith, Correlation between Gd-enhanced MR imaging and histopathology in treated and untreated 9L rat brain tumors Magnetic Resonance Imaging. ,vol. 13, pp. 89- 96 ,(1995) , 10.1016/0730-725X(94)00072-B
J. Bayens-Simmonds, D.P.J. Boisvert, M.E. Castro, E.S. Johnson, A feline model for experimental studies of peritumor brain edema Journal of Neuro-oncology. ,vol. 6, pp. 371- 378 ,(1988) , 10.1007/BF00177435
Myeong Nam, Peter Johnston, Bachchu Lal, Ravinder Indurti, Mary Ann Wilson, John Laterra, Endothelial cell-based cytokine gene delivery inhibits 9L glioma growth in vivo. Brain Research. ,vol. 731, pp. 161- 170 ,(1996) , 10.1016/0006-8993(96)00471-4
Bouvier-Labit, Civatte, Bartoli, Renaud, Pellissier, Figarella-Branger, p16INK4a and p19INK4d mRNA expression in neuroglial tumours: correlation with Ki67 proliferation index Neuropathology and Applied Neurobiology. ,vol. 25, pp. 408- 416 ,(1999) , 10.1046/J.1365-2990.1999.00203.X
Douglas Kondziolka, Salvador Somaza, Christopher Comey, L. Dade Lunsford, Diana Claassen, Sudha Pandalai, Ann Maitz, John C. Flickinger, Radiosurgery and fractionated radiation therapy: comparison of different techniques in an in vivo rat glioma model Journal of Neurosurgery. ,vol. 84, pp. 1033- 1038 ,(1996) , 10.3171/JNS.1996.84.6.1033
Nobuhisa Nagano, Hiroshi Sasaki, Masaru Aoyagi, Kimiyoshi Hirakawa, Invasion of experimental rat brain tumor: early morphological changes following microinjection of C6 glioma cells Acta Neuropathologica. ,vol. 86, pp. 117- 125 ,(1993) , 10.1007/BF00334878
Kenneth G. Rieth, Giovanni Di Chiro, William T. London, John L. Sever, Sidney A. Houff, Paul L. Kornblith, Paul E. McKeever, Carlo Buonomo, Billie L. Padgett, Duard L. Walker, Experimental glioma in primates: a computed tomography model. Journal of Computer Assisted Tomography. ,vol. 4, pp. 285- 290 ,(1980) , 10.1097/00004728-198006000-00001