Development of a xenograft glioma model in mouse brain.

摘要: Abstract Xenograft intracerebral glioma models have been developed in normal mice by growing the rat C 6 either adult or neonatal mouse brains. Using this tumor line it was possible to grow discrete gliomas CBA AKR mice. The size of mass and length survival directly related number cells injected time after implantation. To obtain localized intracranial growth were suspended a 1% agarose solution before Following injection 10 into frontal lobe mice, masses greater than 4 mm diameter obtained 90% animals at 14 days, largest tumors occurred between 21 28 days following implantation significantly with 5 7 ( P cells. found that when intracranially 15 within 2 weeks (CBA mice). Similar results seen RIII, AKR, C57 black, BALB/c strains. Longer periods resulted larger tumors, up 8 (6 20 days). not as often spread meninges lateral ventricles. harvested from brain had cloning efficiency 1.2 ± 0.4% (SD). A panel monoclonal antibodies raised glioma, used define clearly margins brain. xenograft should prove useful for study therapy gliomas.