Tamoxifen-mediated growth inhibition of human cholangiocarcinoma.

摘要: Cholangiocarcinoma represents a challenging primary malignancy of the liver with no effective medical therapy and poor prognosis. We have investigated role tamoxifen estrogen receptors (ERs) in regulation growth human cholangiocarcinoma. Two cholangiocarcinoma cell lines, OZ SK-ChA-1, were grown presence graded concentrations tamoxifen; effects on determined by counting or 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium proliferation assay. The ER protein was tested indirect immunofluorescence immunoprecipitation. In addition, cells estrogen-depleted media supplemented exogenous 17beta-estradiol. mRNA evaluated reverse transcription-PCR Northern blotting. Finally, one line as xenograft athymic nude mice; vivo tumor biweekly caliper measurements. Tamoxifen (5-10 microM) caused dose-dependent vitro inhibition two lines. (SK-ChA-1) mice observed. suggested 17beta-estradiol stimulation confirmed Immunofluorescence microscopy ineffective at detection protein. Reverse demonstrated both blot analysis full-length 6.5-kb mRNA. No deletion mutants detected. inhibited vivo. detected mechanism(s) tamoxifen-mediated is unclear but may occur via additional pathways. ability to inhibit offer an alternative adjunctive treatment for