Productionin vitro of appressoria by the entomopathogenic fungusMetarhizium anisopliae

摘要: Abstract Germination on complex media induced conidia of the entomopathogenMetarhizium anisopliae to produce infection structures (appressoria and penetration hyphae) when germ tube contacted a hard surface. The morphology their rate formation are very similar those observed for blowfly cuticle. Differentiation frequencies were greater (more than 70% as compared with less 40%) hydrophobic surfaces [Teflon, polyvinyl chloride, polystyrene, polypropylene, polyester (GelBond), aluminum foil] hydrophilic (agarose-coated cellophane). both positively negatively charged surfaces. Differentiationin vitro was stimulated by low levels nitrogenous nutrients. Analysis one- or multicomponent suggested that amino acids lipid component epicuticle act in combination cuticle surface stimulate differentiation during pathogenesis. Thigmotropic chemical stimuli production appressoria appear be translated primarily second round nuclear division because inhibitors DNA RNA synthesis do not prevent germination but block if applied before division. Inhibition protein blocked differentiation.