Changes in DNA content of nuclei in rust uredospore germlings during the start of differentiation

作者: R.C. Staples , D. Gross , R. Tiburzy , H.C. Hoch , S. Hassouna

DOI: 10.1016/0147-5975(84)90007-0

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摘要: Abstract Digital video microscopy in conjunction with the DNA-binding fluorescent probe 4′,6-diamidino-2-phenylindole was used to determine relative DNA content of single nuclei germ tubes uredospores bean rust fungus (Uromyces phaseoli), a parasite plants (Phaseolus vulgaris). The develop series infection structures response contact stimuli, and first structure appear, appressorium, occupies stomatal opening. This study made time after start germination on an inductive surface that replication mitosis occur. It found detected by increased nuclear fluorescence power some individual population nearly coincident between 2.0 2.5 h appressorium completed about 30 min later. fluoresence same before began as at end mitosis; hence tube were G1 phase cell cycle mitosis.

参考文章(14)
Ramesh Maheshwari, A. C. Hildebrandt, P. J. Allen, THE CYTOLOGY OF INFECTION STRUCTURE DEVELOPMENT IN UREDIOSPORE GERM TUBES OF UROMYCES PHASEOLI VAR. TYPICA (PERS.) WINT. Botany. ,vol. 45, pp. 447- 450 ,(1967) , 10.1139/B67-045
Clifford F. Brunk, Kenneth C. Jones, Thomas W. James, Assay for nanogram quantities of DNA in cellular homogenates Analytical Biochemistry. ,vol. 92, pp. 497- 500 ,(1979) , 10.1016/0003-2697(79)90690-0
Richard C. Staples, Salah Hassouna, Lucille Laccetti, Harvey C. Hoch, Metabolic alterations in bean rust germlings during differentiation induced by the potassium ion Experimental Mycology. ,vol. 8, pp. 183- 192 ,(1984) , 10.1016/0147-5975(84)90001-X
Richard C. Staples, Synthesis of DNA during differentiation of bean rust uredospores Physiological Plant Pathology. ,vol. 4, pp. 415- 424 ,(1974) , 10.1016/0048-4059(74)90026-5
H. A. Crissman, R. A. Tobey, Cell-Cycle Analysis in 20 Minutes Science. ,vol. 184, pp. 1297- 1298 ,(1974) , 10.1126/SCIENCE.184.4143.1297
Melanie Loo, Some required events in conidial germination of Neurospora crassa. Developmental Biology. ,vol. 54, pp. 201- 213 ,(1976) , 10.1016/0012-1606(76)90299-2