Antiangiogenic Activity and Bioassay-guided Isolation of Aqueous Extract of Orthosiphon stamineus
作者： Khalid Hussain , Zhari Ismail , Amirin Sadikun , Amin Malik Abdual Majid Shah , Abida Latif
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摘要: Orthosiphon stamineus Benth. (Family: Lamiaceae), a famous traditional medicinal plant in tropical and sub-tropical region, is being used for treating various ailments including inflammatory conditions. Since anti-inflammation antiangiogenesis involve cyclooxygenase-II pathway, the present study aimed to carry out antiangiogenic studies bioassay-guided isolation on aqueous extract of plant. After activity extract, fractions were prepared by partitioning with hexane, chloroform ethyl acetate, which then evaluated activity. The most active, hexane fraction (HF) upon activity-guided gave three compounds, 1 (betulinic acid), 2 (oleanolic acid) 3 (ursolic acid). total contents these compounds determined its using HPLC. HF exhibited 20% 80% activity, respectively, whereas, acetate inactive. isolated 100% Median effective concentrations (EC50s) HF, 1, found be 84, 32, 34 38 μg/mL, respectively. results this indicate that have promising Additionally, may sued as analytical markers standardize extracts formulations from O. stamineus.
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