Fluctuations of formamide hydrolase activity in a barley field soil after drying and rewetting

作者: Morten Miller , Tommy Harder Nielsen , Torben Bonde , Jan Sørensen

DOI: 10.1016/S0929-1393(00)00104-9

关键词:

摘要: Abstract Formamide hydrolase activity in a barley field soil was described by two-component, rather than one-component model of Michaelis–Menten kinetics. The two-component had both high-affinity ( K m values 0.5–1.0 mM) and low-affinity 30–60 mM) activity. Rapid transient increases the overall heterotrophic (CO 2 production) component formamide were observed rewetted samples, suggesting that represented enzyme active microorganisms. Short-term variations during growth season consistent with rapid, responses microbial to precipitation wetting. An assay as approximated rate determination at single low (1 mM) concentration can provide an easy rapid indication related directly microorganisms soil.

参考文章(25)
M. Ali, C.R. Jensen, V.O. Mogensen, A. Bahrun, Drought adaptation of field grown wheat in relation to soil physical conditions Plant and Soil. ,vol. 208, pp. 149- 159 ,(1999) , 10.1023/A:1004535819197
Donald J. Cram, Jane M. Cram, The essence of organic chemistry ,(1978)
S. P. Deng, M. A. Tabatabai, Effect of tillage and residue management on enzyme activities in soils. I. Amidohydrolases Biology and Fertility of Soils. ,vol. 22, pp. 202- 207 ,(1996) , 10.1007/BF00382513
Neil R. Wyborn, James Mills, Steven G. Williams, Colin W. Jones, Molecular Characterisation of Formamidase from Methylophilus methylotrophus FEBS Journal. ,vol. 240, pp. 314- 322 ,(1996) , 10.1111/J.1432-1033.1996.0314H.X
Morten Miller, Richard P. Dick, Thermal stability and activities of soil enzymes as influenced by crop rotations Soil Biology & Biochemistry. ,vol. 27, pp. 1161- 1166 ,(1995) , 10.1016/0038-0717(95)00045-G
A. M. McNeill, G. P. Sparling, D. V. Murphy, P. Braunberger, I. R. P. Fillery, Changes in extractable and microbial C, N, and P in a Western Australian wheatbelt soil following simulated summer rainfall Soil Research. ,vol. 36, pp. 841- 854 ,(1998) , 10.1071/S97044
S. P. Deng, M. A. Tabatabai, Effect of tillage and residue management on enzyme activities in soils: III. Phosphatases and arylsulfatase Biology and Fertility of Soils. ,vol. 24, pp. 141- 146 ,(1997) , 10.1007/S003740050222
W.T. Frankenberger, M.A. Tabatabai, Characteristics of an amidase isolated from a soil bacterium Soil Biology and Biochemistry. ,vol. 17, pp. 303- 308 ,(1985) , 10.1016/0038-0717(85)90065-3
W. T. Frankenberger, M. A. Tabatabai, Amidase Activity in Soils: I. Method of Assay Soil Science Society of America Journal. ,vol. 44, pp. 282- 287 ,(1980) , 10.2136/SSSAJ1980.03615995004400020016X