作者: Morten Miller , Tommy Harder Nielsen , Torben Bonde , Jan Sørensen
DOI: 10.1016/S0929-1393(00)00104-9
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摘要: Abstract Formamide hydrolase activity in a barley field soil was described by two-component, rather than one-component model of Michaelis–Menten kinetics. The two-component had both high-affinity ( K m values 0.5–1.0 mM) and low-affinity 30–60 mM) activity. Rapid transient increases the overall heterotrophic (CO 2 production) component formamide were observed rewetted samples, suggesting that represented enzyme active microorganisms. Short-term variations during growth season consistent with rapid, responses microbial to precipitation wetting. An assay as approximated rate determination at single low (1 mM) concentration can provide an easy rapid indication related directly microorganisms soil.