A reduced nicotinamide adenine dinucleotide--linked kinetic assay for adenosine deaminase activity.
作者： David M. Goldberg , Graham Ellis
DOI: 10.5555/URI:PII:0022214370900612
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摘要: Abstract A new kinetic method for measuring the activity of adenosine deaminase (Enzyme Commission 3.5.4.4, aminohydrolase) is described. NH 3 liberated from linked to reduced nicotinamide adenine dinucleotide oxidation by glutamate dehydrogenase 1.4.1.3, l-glutamate:nicotinamide [phosphate] oxidoreductase), and consequent fall in E 340 monitored. Optimum conditions assay were established. Because wavelength at which measurement made, especially suitable crude homogenates serum has general application ammonia-liberating enzymes. Michaelis constant data are given enzymes various tissues human sera normal diseased individuals. range 11.5 25.0 U. per liter. The origin enzyme considered.
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