A purification of adenosine deaminase from the superficial mucosa of calf intestine
作者： T.G. Brady , W. O'Connell
DOI: 10.1016/0006-3002(62)90035-5
关键词: Ultracentrifuge 、 Adenosine deaminase 、 Fractionation 、 Specific activity 、 Enzyme 、 Cellulose 、 Adenosine 、 Biochemistry 、 Isoelectric point 、 Chromatography 、 Chemistry
摘要: Abstract Adenosine deaminase (adenosine aminohydrolase, EC 3·5·4·4) has been purified over 200-fold from acetone-dried calf mucosa by acid and acetone fractionation followed chromatography on DEAE-SF cellulose. The enzyme was obtained in 35–45% yield had a specific activity of 430 units (μmoles adenosine/min at 37°). It homogeneous the ultracentrifuge but separated into four enzymeically active components starch-gel electrophoresis. an isoelectric point pH 4.85 optimum 7.0.
elsevier.com
sci-hub.se 参考文章(24)
Kalckar Hm, DIFFERENTIAL SPECTROPHOTOMETRY OF PURINE COMPOUNDS BY MEANS OF SPECIFIC ENZYMES III. STUDIES OF THE ENZYMES OF PURINE METABOLISM Journal of Biological Chemistry. ,vol. 167, pp. 461- 475 ,(1947) , 10.1016/S0021-9258(17)30999-7
George Brawerman, Erwin Chargaff, On a deoxyribonuclease from germinating barley. Journal of Biological Chemistry. ,vol. 210, pp. 445- 454 ,(1954) , 10.1016/S0021-9258(18)65469-9
M.L. Schaedel, M.J. Waldvogel, F. Schlenk, THE SPECIFICITY OF ADENOSINE DEAMINASE AND PURINE NUCLEOSIDASE Journal of Biological Chemistry. ,vol. 171, pp. 135- 141 ,(1947) , 10.1016/S0021-9258(17)41114-8
Arthur Kornberg, W.E. Pricer, Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside. Journal of Biological Chemistry. ,vol. 193, pp. 481- 495 ,(1951) , 10.1016/S0021-9258(18)50904-2
Marjory Stephenson, Arthur Reginald Trim, The metabolism of adenine compounds by Bact. coli Biochemical Journal. ,vol. 32, pp. 1740- 1751 ,(1938) , 10.1042/BJ0321740
Henry Tauber, Israel S. Kleiner, A METHOD FOR THE DETERMINATION OF MONOSACCHARIDES IN THE PRESENCE OF DISACCHARIDES AND ITS APPLICATION TO BLOOD ANALYSIS Journal of Biological Chemistry. ,vol. 99, pp. 249- 255 ,(1932) , 10.1016/S0021-9258(18)76089-4
Edward Joseph Conway, Microdiffusion Analysis and Volumetric Error ,(1962)
W. E. TREVELYAN, D. P. PROCTER, J. S. HARRISON, Detection of sugars on paper chromatograms. Nature. ,vol. 166, pp. 444- 445 ,(1950) , 10.1038/166444B0
H. Stern, V. Allfrey, A. E. Mirsky, H. Saetren, SOME ENZYMES OF ISOLATED NUCLEI The Journal of General Physiology. ,vol. 35, pp. 559- 578 ,(1952) , 10.1085/JGP.35.3.559
Allan H. Roush, Robert F. Betz, The adenosine deaminase of crustaceans. Biochimica et Biophysica Acta. ,vol. 19, pp. 579- 580 ,(1956) , 10.1016/0006-3002(56)90501-7