Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL

摘要: The ABC transporter ABCA1 regulates HDL levels and is considered to control the first step of reverse cholesterol transport from periphery liver. To test this concept, we studied effect deficiency on hepatic metabolism hepatobiliary flux in mice. Hepatic lipid contents biliary secretion rates were determined Abca1(-/-), Abca1(+/-), Abca1(+/+) mice with a DBA background that fed either standard chow or high-fat, high-cholesterol diet. phospholipid Abca1(-/-) indistinguishable those Abca1(+/-) both diets. In spite absence HDL, cholesterol, bile salts, unimpaired Neither expression genes controlling key steps nor contribution de novo synthesis salts affected by Abca genotype. Finally, fecal excretion neutral acidic sterols was similar all groups. We conclude plasma activity do not net via liver into bile, indicating importance requires re-evaluation.