Quantitative evaluation of serum pancreatic isoamylases in cystic fibrosis.

摘要: The amylase in sera of 20 patients with cystic fibrosis the pancreas (CFP), 17 whom had pancreatic insufficiency (PI), and 34 normal control subjects was separated into its salivary components quantitated. Electrophoretic separation achieved on polyacrylamide disc gels subsequent sectioning gel columns. content international units per liter (IU/L.) obtained for each section by insoluble blue starch substrate method. mean total serum CFP PI significantly lower than group. A highly significant decrease found when isoamylase considered alone. level remained essentially same. Three but without demonstrated higher values serum. These may be an early stage a progressive involvement which there is increased release amylase. This method electrophoretic quantitation sensitive efficient allows simultaneous processing multiple specimens. results agree previously published ratios isoamylases. Our should valuable study other diseases exocrine glands.