Biochemical characterization of the lysosomes of ehrlich ascites tumor cells

摘要: Abstract 1. The presence of lysosome-like particles in Ehrlich ascites tumor cells has been demonstrated by the use biochemical methods. intracellular distribution pattern 4 lysosomal marker enzymes (deoxyribonuclease II, aryl sulfatase, acid phosphotase, and β-galactosidase) demonstration latency these were two major criteria applied to identification organelles particulate fraction obtained differential centrifugation. Studies on physical properties lysosomes revealed an increased stability as compared similar rat liver kidney. cell are further distinguished from those liver, for example, having a lower density than mitochondria, being denser mitochondria. 2. A partial purification deoxyribonuclease II was undertaken because contradictory reports literature concerning enzyme cells. results show that is indeed present displays characteristics found normal tissues.