Detection of point mutations in K-ras gene at codon 12 in bile from percutaneous transhepatic choledochal drainage tubes for diagnosis of biliary strictures.

摘要: Detection of K-ras mutations at codon 12 constitutes one modality for diagnosis pancreatic tumors. We attempted to detect in DNA from bile collected through percutaneous transhepatic choledochal drainage (PTCD) tubes as a diagnostic approach biliary strictures. Since salts induce cell damage, we first investigated the degeneration cells according exposure time using lines. High-mol-wt could be extracted exposed 6 h, but not those h. However, up h amplified by polymerase chain reaction (PCR) method. Therefore, fresh specimens 15 patients PTCD were examined PCR with restriction enzyme digestion. found five out (100%) cancers, all which negative cytodiagnosis same bile. On other hand, detected tract cancers or metastatic neoplasms, except duct carcinoma and case. Thus, although mutation alone is an absolute marker cancer, detection useful adjunct carcinomas cases