Frequent c-Ki-ras Oncogene Activation in Mucous Cell Hyperplasias of Pancreas Suffering from Chronic Inflammation

摘要: In order to scrutinize the possible significance of (nonatypical) mucous cell hyperplasia pancreas neoplasia, we analyzed these lesions in terms c-Ki- ras activation, which is known be very frequent pancreatic carcinomas. A total 16 such hyperplasias were collected from 10 pancreases resected for chronic pancreatitis. Tiny tissue fragments taken hematoxylin-stained sections by microdissection, and DNA analysis was carried out polymerase chain reaction amplification oligonucleotide hybridization methods. Activating mutations oncogene at codon 12 detected (62.5%), a high rate as seen The results indicated clonal origin cells comprising suggesting neoplastic and/or precancerous nature.