Pseudoverification of mixed aminoacyl transfer ribonucleic acids. The generality of the process.

摘要: Abstract A mixture of unresolved tRNAs from Escherichia coli B has been acylated with a 18 3H-amino acids, and the purified, correctly aminoacyl-tRNAs were incubated pure isoleucyl-tRNA synthetase under conditions in which it hydrolyzes some noncognate (see preceding paper). micromethod was used to resolve identify acids released. The results indicate that can interact deacylate 15 aminoacyl-tRNA species used. occurrence this reaction indicates an interaction between these synthetase. In addition, specificity verification, or hydrolysis, is illuminated by particular nature residues are resistant hydrolysis.