Morphometry and cell proliferation in endoscopic biopsies: evaluation of a technique.

作者: Robert A. Goodlad , Sass Levi , Chung Y. Lee , Nirmala Mandir , Humphrey Hodgson

DOI: 10.1016/0016-5085(91)90072-S

关键词:

摘要: Abstract A simple method for the quantification of intestinal epithelial cell proliferation in humans was evaluated. Endoscopic biopsy specimens were stained and microdissected, number mitoses per gland or crypt determined, as area these units. The technique could readily be applied to tissue from stomach, small intestine colon, rectum. size proliferative compartments increased caudally stomach cecum humans. There a good correlation between ( r = 0.89; P

参考文章(30)
Malcolm Alison, Nicholas A. Wright, The biology of epithelial cell populations Clarendon Press , Oxford University Press. ,(1984)
Nicholas A. Wright, William A. Aherne, Richard S. Camplejohn, An introduction to cell population kinetics ,(1977)
R.A. Goodlad, A.J. Madgwick, M.R. Moffatt, S. Levin, J.L. Allen, N.A. Wright, Effects of misoprostol on cell migration and transit in the dog stomach. Gastroenterology. ,vol. 98, pp. 90- 95 ,(1990) , 10.1016/0016-5085(90)91295-H
I.F. Tannock, A comparison of the relative efficiencies of various metaphase arrest agents Experimental Cell Research. ,vol. 47, pp. 345- 356 ,(1967) , 10.1016/0014-4827(67)90237-6
A Ferguson, A Sutherland, T T MacDonald, F Allan, Technique for microdissection and measurement in biopsies of human small intestine. Journal of Clinical Pathology. ,vol. 30, pp. 1068- 1073 ,(1977) , 10.1136/JCP.30.11.1068
S Levi, M.J Walport, H.J.F Hodgson, R.A Goodlad, C.Y Lee, G Stamp, N.A Wright, Inhibitory effect of non-steroidal anti-inflammatory drugs on mucosal cell proliferation associated with gastric ulcer healing. The Lancet. ,vol. 336, pp. 840- 843 ,(1990) , 10.1016/0140-6736(90)92341-E
Hazel Cheng, Matthew Bjerknes, Jack Amar, Geoffrey Gardiner, Crypt production in normal and diseased human colonic epithelium Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology. ,vol. 216, pp. 44- 48 ,(1986) , 10.1002/AR.1092160108
D. Wynford-Thomas, E. D. Williams, Use of Bromodeoxyuridine For Cell Kinetic Studies In Intact Animals Cell Proliferation. ,vol. 19, pp. 179- 182 ,(1986) , 10.1111/J.1365-2184.1986.TB00728.X
R.A. Goodlad, A.J. Madgwick, M.R. Moffatt, S. Levin, J.L. Allen, N.A. Wright, The effects of the prostaglandin analogue, misoprostol, on cell proliferation and cell migration in the canine stomach Digestion. ,vol. 46, pp. 182- 187 ,(1990) , 10.1159/000200384