Ecarin Clotting Time is Sensitive to Heparinoids: Comparison of Two Different Techniques

摘要: Ecarin clotting time (ECT) is currently developed for the specific monitoring of antithrombin drugs, such as hirudin, argatroban, and hirulog. Aqueous reagent dry chemistry technology have become available ECT agents. Currently, many heparinoids heparinomimetic drugs are being developed. These agents activate heparin cofactor II (HCII) primarily mediate their effects by inhibiting thrombin. Although test agents, II-mediated thrombin inhibitors capable prolonging ECT. In order to study relative some these was measured in human plasma supplemented with Pl-88 (a sulfated pentomanose; Progen Industries Limited, Sydney, Australia), aprosulate, pentosan polysulfate, dermatan sulfate, unfractionated (UFH), recombinant hirudin (r-hirudin). All were citrated-pooled prepared from 10 healthy volunteers at a graded dosage 0 100 microg/ml. techniques gave comparable results all used (PI-88, r2 = 0.99; r-hirudin, 0.98; UFH, 0.95; 0.94). The anticoagulant various on varied widely, exhibiting potency following order: r-hirudin polysulfate > sulfate PI-88 aprosulate UFH. sensitivity adjusted varying concentration ecarin reagent. suggest that HCII-mediated inhibition can be detected using reagents.