Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks

摘要: A thorough characterization of the transcriptome and proteome endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol efficient single dissociation to yield more than 500,000 per allowing for global, unbiased downstream applications. Combining mRNA miRNA transcriptional profiling quantitative proteomic analyses revealed programs highly specific gene regulation tightly controlling cytoskeleton, differentiation, endosomal transport, peroxisome function in podocytes. Strikingly, further predict that these podocyte-specific regulatory networks are accompanied alternative splicing respective genes. Thus, our ‘omics' approach will facilitate discovery integration novel gene, protein, organelle deepen systematic understanding podocyte biology.