Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*.

摘要: Abstract Rev-erb β is an orphan receptor that binds as a homodimer or monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), response element for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints distances NOE back-calculation methods. refined final structures have typical overall features B-type However, titration this ReDBD, binding domain β, showed large shifts imino protons signals, suggesting major conformational changes.