Differential gene activation by glucocorticoids and progestins through the hormone regulatory element of mouse mammary tumor virus

摘要: Abstract The hormone regulatory element (HRE) of mouse mammary tumor virus can mediate activation an adjacent promoter by glucocorticoids and progestins. A detailed comparison the DNA binding receptors for both hormones using DNAase I footprinting methylation protection detects clear differences in their interactions with HRE region between positions −130 −100. Binding studies gene transfer experiments a variety mutants covering entire demonstrate relevance individual sequence motifs induction each hormone. influence changes angular orientation receptor sites is also different glucocorticoid progesterone induction. In transfection mutated HREs, we find functional cooperation that does not correlate variations vitro affinity corresponding fragment.