Chromosomal aneuploidy precedes morphological changes and supports multifocality in head and neck lesions.

摘要: Objective To identify chromosome changes associated with the transformation of dysplastic lesions and to verify evidence for multifocality in synchronous premalignant head neck squamous cell carcinoma (HNSCC). Study Design Chromosomal aneuploidy was evaluated sections formalin-fixed, paraffin-embedded tissues from 16 patients HNSCC, including sites normal mucosa, dysplasia (low- high-grade), invasive tumor. Methods A panel 6 centromeric probes (chromosomes 1, 3, 7, 8, 9, 17) analyzed dual-color fluorescence situ hybridization assays, using matched hematoxylin–eosin-stained histologic correlation. Results Imbalances most targets tested were found 20 24 sites, mainly represented by gain copy number per cell. However, populations losses gains multimodal patterns concomitantly observed a tumors, indicating high degree instability. The detection chromosomal precedes malignant as indicated findings monosomy trisomy low-grade high-grade sites. Loss chromosomes 3 17 prevailed dysplasias, 7 8 prevalent dysplasias. Synchronous displayed discordant molecular signatures, suggesting multifocal origin. Conclusions interphase in-situ (FISH) assay may detect early progression dyplastic epithelia supports field cancerization theory multifocality.