Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain
作者： Jiankun Cui , Eric H. Holmes , Thomas G. Greene , Philip K. Liu
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摘要: Experimental stroke using a focal cerebral ischemia and reperfusion (FCIR) model was induced in male Long-Evans rats by bilateral occlusion of both common carotid arteries the right middle artery for 30–90 min, followed various periods reperfusion. Oxidative DNA lesions ipsilateral cortex were demonstrated Escherichia coli formamidopyrimidine N-glycosylase (Fpg protein)-sensitive sites (FPGSS), as labeled situ digoxigenin-dUTP detected antibodies against digoxigenin. Because Fpg protein removes 8-hydroxy-2′-deoxyguanine (oh8dG) other DNA, FPGSS measure oxidative damage. The number FPGSS-positive cells from sham-operated control group 3 ± (mean sd per mm2). In animals that received 90 min 15 (FCIR 90/15), significantly increased 200-fold. damage confirmed monoclonal 8-hydroxy-guanosine (oh8G) ...
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