Mechanism for antiplatelet effect of onion: AA release inhibition, thromboxane A2synthase inhibition and TXA2/PGH2receptor blockade
作者： C.H. Moon , Y.S. Jung , M.H. Kim , S.H. Lee , E.J. Baik
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摘要: Antiplatelet actions of aqueous extract onion were investigated in rat and human platelet. IC(50)values for collagen-, thrombin-, arachidonic acid (AA)-induced aggregations collagen-induced thromboxane A(2)(TXA(2)) formation 0.17 +/- 0. 01, 0.23 + 0.03, 0.34 0.02 0.12 0.01 g/ml, respectively. [(3)H]-AA release induced by collagen (10 microg/ml) platelet was decreased compared to control (22.1 2.13 5.2 0.82% total incorporated, respectively). In fura-2 loaded platelets, the elevation intracellular Ca(2+)concentration stimulated inhibited onion. Onion had no cytotoxic effect significantly TXA(2)synthase activity without influence on COX activity. Platelet aggregation U46619, a stable TXA(2)mimetic, onion, indicating its antagonism TXA(2)/PGH(2)receptor. These results suggest that mechanism antiplatelet may, at least partly, involve AA diminution, inhibition TXA(2)/PGH(2)receptor blockade.
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