A 35 kDa antigenic protein from Shigella flexneri: In silico structural and functional studies

摘要: Shigella flexneri serotype 2a is a major public health concern in the developing and under-developed countries which contributes to shigellosis endemic mortality. Thus, there an urgent need for rapid diagnostic test effective therapy disease management. Previous study showed that ∼35 kDa antigenic protein from S. potential biomarker. We therefore modelled three-dimensional structure of antigen probe its functionality could aid development antigen-based diagnostic. Results transmembrane consists OmpA OmpA-like domains. The domain beta-barrel embedded outer membrane with four surface-exposed extracellular loops. linked 17 amino acids linker located periplasmic. Docking peptidoglycan into groove might help catalyzing bacterial cell wall formation. Both domains are expected be involved virulence, structural stability, pathogenesis survival thus made 35 suitable This elucidation will also enable better identification epitope regions specific binders antigen.