Induction of immune response to the 17 kDa OMPA Burkholderia cenocepacia polypeptide and protection against pulmonary infection in mice after nasal vaccination with an OMP nanoemulsion-based vaccine.

摘要: Burkholderia cepacia complex (Bcc) are opportunistic bacteria associated with life-threatening illness in persons cystic fibrosis. Once Bcc colonization is established, these antimicrobial-resistant and biofilm-forming difficult to eradicate increased rates of morbidity mortality. At present, no vaccines available prevent the infection. There currently a paucity published information regarding development designed colonization. This work expands on recent studies by Bertot et al. [Infect Immun 75(6):2740–2752, 2007], where successful protective immune responses were generated mice using B. multivorans OMP-based vaccine. Here, we evaluate an experimental mucosal vaccine against novel adjuvant (nanoemulsion) cenocepacia-based OMP antigen. The antigen derived from cenocepacia was mixed either nanoemulsion or PBS delivered intranasally CD-1 mice. Serum analysis showed robust IgG secretory IgA vaccinated versus control antibodies had cross-neutralizing activity both species. We found that immunized protected pulmonary cenocepacia. have also identified 17 kDa OmpA-like protein highly conserved between Ralstonia species as new immunodominant epitope immunization.