Protein expression via seed specific regulatory sequences
作者： Paula Po Chee , Jerry Laverne Slightom
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摘要: Cassettes d'expressions specifiques de semences permettant la regulation et l'expression, dans certaines plantes, proteines importantes pour les besoins pharmacologiques industriels. specific seed expressions for the and expression, in some plants, proteins important pharmacological industrial needs. On peut construire rapidement ces cassettes en joignant des elements regulateurs par l'intermediaire sites enzymatiques restriction synthetises produire oligomeres d'amplification utilises afin cloner regulateurs. These can quickly build enclosing regulatory via enzymatic synthesized to produce amplification oligomers used clone sites. Selon un procede l'invention, region d'activation du gene phaseoline est fixee a intron codant somatotropine bovine (Bst), elle liee signal poly-A provenant phaseoline, Bst, ou deux, permettre transfert genome plantes. According one method of invention, activation phaseolin is attached an encoding somatotropin (BST), it linked from Bst or both, allow transfer plant genome.
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