Tanshinone IIA pretreatment protects free flaps against hypoxic injury by upregulating stem cell-related biomarkers in epithelial skin cells

摘要: Partial or total flap necrosis after transplantation is sometimes encountered in reconstructive surgery, often as a result of period hypoxia that exceeds the tolerance tissue. The purpose this study was to determine whether Tanshinone IIA (TSA) pretreatment can protect tissue against hypoxic injury and improve its viability. Primary epithelial cells isolated from dorsal skin mice were pretreated with TSA for 2 weeks. Cell Counting Kit-8 Trypan Blue assays carried out examine proliferation TSA-pretreated exposure cobalt chloride. Polymerase chain reaction western blot analysis used assess expression β-catenin, vascular endothelial growth factor (VEGF), sex determining region Y-box 2 (SOX2), OCT4 (also known POU domain class 5 transcription 1), Nanog, glycogen synthase kinase-3 beta (GSK-3β) TSA-treated cells. In other experiments, 2 weeks, reproducible ischemic model implemented, area surviving transplanted flaps measured. Immunohistochemistry conducted Wnt signaling well stem cell- angiogenesis-related biomarkers vivo. Epidermal cells, TSA, showed enhanced resistance hypoxia. Activation pathway characterized by upregulation β-catenin downregulation GSK-3β. SOX2, also higher than control model, increased flaps. components, stem-cell related biomarkers, VEGF CD34, which are involved regeneration blood vessels, upregulated protects free increases activating upregulating cell-related biomarkers.