High-pressure homogenization for the inactivation of human enteric virus surrogates.

摘要: Novel inactivation methods are needed to control the spread of foodborne viruses responsible for nonbacterial gastroenteritis worldwide. The advent high-pressure homogenization combining high pressure, shear stress, and cavitation provides opportunity evaluate this technology viral in fluid foods under continuous processing conditions. Our objective was murine norovirus (MNV-1) MS2 coliphage (single-stranded RNA) as human enteric virus surrogates their susceptibility a novel process application commercial settings. Experiments were conducted duplicate with MNV-1 phosphate-buffered saline, using pressures 0, 100, 200, 250, 300 MPa (the maximum achievable by homogenizer), resulting exposure temperatures 24, 46, 63, 70, 75°C, respectively, <2 s. Only at 75°C showed ~3 log PFU from an initial ~6 PFU...