Effect of retinoic acid on human neuroblastoma: correlation between morphological differentiation and changes in plasminogen activator and inhibitor activity.

摘要: The relationship between plasminogen activator (PA)/plasminogen inhibitor (PAI) activity and morphological differentiation was investigated in human neuroblastoma (NB) cells treated with retinoic acid (RA). Conditioned medium from nine NB cell lines one closely related neuroepithelioma line analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) zymography. All were shown to secrete urokinase (UK)-type PA (mol. wt., 52 kDa), all except two produced tissue 65 kDa). Identification of the PAs made based on molecular weight sensitivity inhibition anti-UK anti-tPA antibodies. Several expressed inhibitory molecules; molecular-weight forms observed (35 40 kDa) different lines. Complex formation [125]I-labelled proteases revealed specific binding UK trypsin but not thrombin, plasmin, or kallikrein. After treatment for 6 days 1 microM RA, six exhibited an increase cell-associated and/or secreted tPA activity, corresponding as manifested extensive neurite outgrowth. A decrease UK-complex secretion several these Three exhibiting no detectable alterations RA also showed dramatic changes PA/PAI activity. These results suggest that may be associated regulation