Osteoblast response to zirconia modified-ORMOSILs.

摘要: In this study, an in vitro evaluation of the human osteoblasts response to Organically Modified Silicate (ORMOSIL) biomaterials was conducted. These materials were synthetized by sol-gel process being modified with zirconia (ZrO2) and/or Ca2+. The immersed into phosphate buffer solution (PBS) order test precipitation mimetic apatite-like on their surfaces. ORMOSILs characterized SEM, FT-IR and X-RD analysis. osteoblast analyzed as a measure cell adhesion, proliferation differentiation. results showed that addition Ca2+ ions modifies surface morphology forming precipitates cauliflower scales morphologies. On other hand, biological suggest incorporation increases ability support adhesion proliferation. However, inclusion both decreases compatibility showing less lower osteonectin expression, protein related osteoblasts. unfavorable effect viability could be due its induce formation incompatible morphology. analysis proteins bone ORMOSIL-Zr ORMOSIL-Zr-Ca surfaces demonstrated clear expression osteopontin osteocalcin cells growth. case ORMOSIL-Zr, occurred at early stages while begun later stages, indicating switch from mature stage stimulated biomaterial. Together, these highlight important role composition regulating biocompatibility when used scaffolds regeneration.